If it is new user registration you can insert the values in database and if the user id already available you can update the values. use bellow code insert values
private void Insert()
{
SqlConnection cn=new SqlConnection("Your String);
SqlCommand cmd=new SqlCommand("Insert Into YourTable(UserName,Password,Authentication)Values('"+txtuname.text+"','"+txtpwd.Text
+"','"+combobox.SelectedItem.Text+"')",cn);
cn.Open();
int _records=cmd.ExecuteNonQuery();
cn.Close();
}
if it is update then change the command like update table set column=value like you can.