If you've created an Application Domain and
want to unload all the assemblies loaded within, there is no way to unload
Assembly themselves. But you can unload Application Domain you've just created.
In addition to that one;
You cannot unload the default Application Domain CLR created on its own. And
unloading can differ from the assemblies being used and the unload events these
assemblies have. It can take some time.
To Unload an Application Domain all you need to do is calling Unload function.
A sample code can help you understand:
you loaded your assemblies
AppDomain call Unload Function
Application Domain we've just created is now no more as the assemblies with it.
After Unloading Application Domain object, we've also unloaded all the
assemblies loaded within.