I have use UpdateProgress for display loader first time loader display properly and after finish loading data ,data will display properly but second time when i click on loader or anywhere in screen data not reflected in gridview.
<asp:UpdateProgress ID="UpdateProgress1" runat="server" AssociatedUpdatePanelID="UpdatePanel1">
<ProgressTemplate>
<div class="divWaiting">
<asp:Image ID="imgWait" runat="server" ImageAlign="Middle" ImageUrl="~/Content/Images/loader.gif" />
</div>
</ProgressTemplat>
</asp:UpdateProgress>