IIS Folder or File Permission Issue

Hi,

 

I've hosted my web application into IIS web server. Using that hosted application the users can create an audio file dynamically. After created that file there is an option to play that file. At the time of playing that file i got some of the issues looks below.

 

1.Access is denied. (Exception from HRESULT: 0x80070005 (E_ACCESSDENIED))

2.Value does not fall within the expected range.

3.Attempted to access an unloaded appdomain. (Exception from HRESULT: 0x80131014) 

 

   I've given the full access rights to IIS_User and authendicated users  for those Folder which have that created Audio files.

 

 

Thanks in advance.