file .txt in python

Question

Hi, I'm looking to make an application for myself that will allow me to find my password I have between my email and my password to save it then it prevents me to write my email and I re-read my password but I can not put more email and password in my text file if I enter another email and password its primed me the other email and word Pass.

def inscription_code():
    email_inscription = email_entry.get()
    pass_inscription = pass_entry.get()
    File_inscription_name = os.listdir()
 if str(email_inscription) + ".txt" in File_inscription_name:
 print("email deja utiliser")
 else:
        File = open(str(email_inscription) + ".txt", "w")
        File.write(str(email_inscription) + ":" + str(pass_inscription))
        File.close()

def File_read():
    File = open()
    File.read()
 print(File)
    File.close()

thank you in advance.